Enzyme-linked immunosorbent assay for free thyroxin in human serum.
نویسندگان
چکیده
We describe a simple enzyme-linked immunosorbent assay (ELISA) in which microtiter plates are used for determining the free thyroxin concentration (FT4) in serum. Only commercially available chemicals and reagents are needed, including the antiserum. The working range is 1 to 60 ng/L. Turnaround time is about 4 h. Within-run coefficients of variation (CV) for FT4 concentrations of 9.0, 19.0, and 35.0 ng/L were less than 5%; between-run CVs were 10% to 11%. Results by a radioimmunoassay method for 150 sera correlated well (r = 0.922).
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 35 8 شماره
صفحات -
تاریخ انتشار 1989